Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions

Objectives: To evaluate DNA ploidy and S-phase fraction (SPF) in non-Lynch colonic adenocarcinoma, ulcerative colitis (UC), Crohn disease (CD) which are known as risk factors, colitis. We correlated SPF with tumor grading, staging BRAF expression. Methods: All studied adenocarcinomas have intact mismatch repair genes proved by immunohistochemistry. were assessed for automated image-based cytometry histograms drawn. Immunostaining anti-BRAF V600E was performed. Diagnostic laparoscopy (DL) done a preliminary step GI cancers. Results: there is significant difference between groups; 77.5% 17.5% of aneuploid cases adenocarcinoma UC. Groups compared terms 2C, 4C, above 4C content principally found group others. In adenocarcinomas, significantly grading. Regarding expression, all 83.33% UC positive, while colitis, bilharzial CD negative. There relation among diploid including non-neoplastic cases. direct intensity staging. no cases, although 75% positive. DL helps cancer Routine before laparotomy, especially cancers equivocal operability to avoid unnecessary laparotomies. Conclusion: Based on ploidy, assessment other could therefore predict impending malignant transformation development dysplasia. Also measuring select patients who greatly benefit from chemotherapy. has vital role